HEG1 Rabbit Polyclonal Antibody

• Catalog Number: orb157480
• Category: Antibodies
• Description: HEG1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HEG1 (1151-1381/1381aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 144 kDa
• Target: HEG1
• UniProt ID: Q9ULI3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HEG; HEG1; HEG1_HUMAN; KIAA1237; MST112; MSTP112;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Liu, Yangzhou et al. MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13 Front Oncol, 11, 754353 (2021)

PubMed: 34722307

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HEG homolog 1) Polyclonal Antibody, Unconjugated (orb157480) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868589) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HEG homolog 1) Polyclonal Antibody, Unconjugated (orb157480) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.