HECW1 Polyclonal Antibody

• Catalog Number: orb1421036
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HECW1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P
• Reactivity: Rat
• Conjugation: Unconjugated
• UniProt ID: Q76N89
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: E3 ubiquitin-protein ligase HECW1, 2.3.2.26, HECT,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HECW1 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.