HECTD1 Antibody

SKU: orb1255618

Description

HECTD1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2518-2610 of human HECTD1 (NP_056197.3).
• Target: HECTD1
• Molecular Weight: Observed: 300kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EULIR, E3 ubiquitin-protein ligase HECTD1, E3 ligase for inhibin receptor, HECT domain containing E3 ubiquitin protein ligase 1, HECT domain-containing protein 1, HECT-type E3 ubiquitin transferase HECTD1

Western blot analysis of extracts of various cell lines, using HECTD1 antibody (orb1255618) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Quick Database Links

Gene Symbol

HECTD1

UniProt

Q9ULT8