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Catalog Number | orb1255618 |
---|---|
Category | Antibodies |
Description | HECTD1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 2518-2610 of human HECTD1 (NP_056197.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 300kDa |
Target | HECTD1 |
UniProt ID | Q9ULT8 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | EULIR, E3 ubiquitin-protein ligase HECTD1, E3 liga Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HECTD1 antibody (orb1255618) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
ELISA, ICC, IF, IHC-P | |
Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
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