HECT E3 ubiquitin ligase Rabbit Polyclonal Antibody

SKU: orb157475

Description

HECT E3 ubiquitin ligase Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HECT E3 ubiquitin ligase (1-100/1024aa)
• Target: HERC5
• Molecular Weight: 117 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CEB1; CEBP1; Cyclin E binding protein 1; Cyclin-E-binding protein 1; E3 ISG15--protein ligase HERC5; HECT domain and RCC1 like domain containing protein 5; HECT domain and RCC1-like domain-containing protein 5; Hect domain and RLD 5; HECT E3 ubiquitin ligase; HERC5; HERC5_HUMAN; Herc6.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HECT E3 ubiquitin ligase) Polyclonal Antibody, Unconjugated (orb157475) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HECT E3 ubiquitin ligase) polyclonal Antibody, Unconjugated (orb157475) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: Rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CEB1 Polyclonal Antibody, Unconjugated (orb157475) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

HERC5

UniProt

Q9UII4