HECT E3 ubiquitin ligase Rabbit Polyclonal Antibody

• Catalog Number: orb157475
• Category: Antibodies
• Description: HECT E3 ubiquitin ligase Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Rat
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HECT E3 ubiquitin ligase (1-100/1024aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 117 kDa
• Target: HERC5
• UniProt ID: Q9UII4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CEB1; CEBP1; Cyclin E binding protein 1; Cyclin-E-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HECT E3 ubiquitin ligase) Polyclonal Antibody, Unconjugated (orb157475) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HECT E3 ubiquitin ligase) polyclonal Antibody, Unconjugated (orb157475) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: Rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CEB1 Polyclonal Antibody, Unconjugated (orb157475) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.