Hec1 antibody

• Catalog Number: orb767343
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Hec1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human KNTC2. AA range:351-400
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 73
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NDC80 antibody, anti-HEC antibody, anti-HEC1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, Hec1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Kif 7 Monoclonal Antibody (3F8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of Jurkat cells using Hec1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of HUVEC cells, using KNTC2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using KNTC2 Antibody. The lane on the right is blocked with the synthesized peptide.