HEC1/HEC/NDC80 Antibody

• Catalog Number: orb614129
• Category: Antibodies
• Description: HEC1/HEC/NDC80 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human HEC1/HEC/NDC80 recombinant protein (Position: N87-E602).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 74 kDa
• UniProt ID: O14777
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Kinetochore protein NDC80 homolog; Highly expresse
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IF analysis of NDC80 using anti-NDC80 antibody.

IF analysis of NDC80 using anti-NDC80 antibody.

WB analysis using anti-NDC80 antibody.Lane 1:K562 cell, Lane 2:rat spleen tissue, Lane 3:mouse spleen tissue.

IF analysis of NDC80 using anti-NDC80 antibody.

Flow Cytometry analysis of SiHa cells using anti-NDC80 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HL-60 cells using anti-NDC80 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.