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HDGF Antibody (C-term)
Catalog Number: orb1787923
| Catalog Number | orb1787923 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This HDGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-197 amino acids from the C-terminal region of human HDGF. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | 171-197 aa |
| UniProt ID | P51858 |
| MW | 26788 Da |
| Tested applications | IF, IHC-P, WB |
| Dilution range | IF: 1:10~50, WB: 1:2000, IHC-P: 1:10~50 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Hepatoma-derived growth factor, HDGF, High mobilit Read more... |
| Note | For research use only |
| NCBI | NP_001119522.1, NP_004485.1, NP_001119523.1 |
Confocal immunofluorescent analysis of HDGF Antibody (C-term) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-HDGF Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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