HDAC4 Antibody

• Catalog Number: orb18862
• Category: Antibodies
• Description: HDAC4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human HDAC4 recombinant protein (Position: Q4-Q213). Human HDAC4 shares 98.6% and 98.1% amino acid (aa) sequence identity with mouse and rat HDAC4, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 119040 MW
• UniProt ID: P56524
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Histone deacetylase 4;HD4;3.5.1.98;HDAC4;KIAA0288;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-HDAC4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of SiHa cells using anti-HDAC4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of HDAC4 using anti-HDAC4 antibody.Lane 1:human HEK293 cell; 2:human HELA cell; 3:human THP-1 cell; 4:human HEPG2 cell; 5:human U87 cell; 6:rat testis tissue; 7:mouse testis tissue; 8:mouse NIH/3T3 cell.

IF analysis of HDAC4 using anti-HDAC4 antibody. HDAC4 was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of HDAC4 using anti-HDAC4 antibody. HDAC4 was detected in paraffin-embedded section of human bladder cancer tissue.

IHC analysis of HDAC4 using anti-HDAC4 antibody. HDAC4 was detected in paraffin-embedded section of human pancreatic cancer tissue.