HDAC4 Antibody (C-term)

SKU: orb31802

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Cardiovascular Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10-50, IF/ICC: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB33667
• Immunogen: Synthetic Peptide
• Target: HDAC4
• Molecular Weight: 119040
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Anti-Histone deacetylase 4 antibody, anti-HDAC4 antibody, anti-KIAA0288 antibody

Fluorescent confocal image of Hela cell stained with HDAC4 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.

HDAC4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HDAC4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HDAC4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35 ug/lane). This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).

Quick Database Links

Gene Symbol

HDAC4

UniProt

P56524

RefSeq (Protein):NP_006028.2