HDAC3 Antibody

• Catalog Number: orb546298
• Category: Antibodies
• Description: HDAC3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human HDAC3 recombinant protein (Position: M1-I428).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Flow Cytometry, 1-3 μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 49 kDa
• UniProt ID: O15379
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Histone deacetylase 3; HD3; RPD3-2; SMAP45; HDAC3
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of MCF-7 cells using anti-HDAC3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of RAW264.7 cells using anti-HDAC3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of RH35 cells using anti-HDAC3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-HDAC3 antibody.Lane 1:293T cell; 2:HeLa cell; 3:HepG2 cell; 4:human MOLT-4 cell; 5:human MCF-7 cell; 6:human K562 cell; 7:human CACO-2 cell; 8:rat thymus tissue; 9:rat C6 cell; 10:mouse ANA-1 cell; 11:mouse NIH/3T3 cell.