HAVCR2/TIM-3 Mouse Monoclonal Antibody

• Catalog Number: orb783345
• Category: Antibodies
• Description: HAVCR2/TIM-3 Mouse Monoclonal Antibody
• Target: HAVCR2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b,k
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human HAVCR2.
• UniProt ID: Q8TDQ0
• MW: 31 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1:25
• Antibody Type: Primary Antibody
• Clone Number: H9F4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TIM-3; Tim 3; TIM3; TIMD-3; TIMD3; HAVR2_HUMAN; He
• Note: For research use only

Blank control: U-2 OS. Primary Antibody (green line): Mouse Anti-HAVCR2 antibody (orb783345), dilution: 1:25, Isotype Control Antibody (blue line): Mouse IgG, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1:200, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: HepG2 cell lysates, Lane 2: Daudi cell lysates, Lane 3: Human Kidney tissue lysates, Primary: Anti-HAVCR2 (orb783345) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 27 kD.