HAVCR2/TIM-3 Mouse Monoclonal Antibody

• Catalog Number: orb2563483
• Category: Antibodies
• Description: HAVCR2/TIM-3 Mouse Monoclonal Antibody
• Target: HAVCR2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, κ
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human TIM-3 protein (22-200/301aa)
• UniProt ID: Q8TDQ0
• MW: 33 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:100-500, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TIM-3; Tim 3; TIM3; TIMD-3; TIMD3; HAVR2_HUMAN; He
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (HAVCR2) Monoclonal Antibody, Unconjugated (orb2563483) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (HAVCR2) Monoclonal Antibody, Unconjugated (orb2563483) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Recombinant human TIM-3 protein, His (HEK293), Primary: Anti-HAVCR2/TIM-3 (orb2563483) at 1/1000 dilution, Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific, Predicted band size: 33 kDa, Observed band size: 40 kDa.