HAS2 Rabbit Polyclonal Antibody

SKU: orb157430

Description

HAS2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HAS2/Hyaluronan synthase 2 (401-500/552aa)
• Target: HAS2
• Molecular Weight: 64 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

HA synthase 2; HAS2_HUMAN; Hyaluronan synthase 2; Hyaluronate synthase 2; Hyaluronic acid synthase 2.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-HAS2 antibody (orb157430), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-HAS2 antibody (orb157430), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Huh7 Cell (Human) Lysate at 40 ug, Primary: Anti-HAS2 (orb157430) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 64 kD, Observed band size: 64 kD.

Sample: Lane 1: Human U251 cell Lysates, Lane 2: Human U-87 MG cell Lysates, Lane 3: Human Hela cell Lysates, Primary: Anti-HAS2 (orb157430) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64kDa, Observed band size: 60kDa.

Quick Database Links

Gene Symbol

HAS2

UniProt

Q92819

References

Reiprich, Sebastian et al. Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 22, (2021)

PubMed: 33803805