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Catalog Number | orb2637207 |
---|---|
Category | Antibodies |
Description | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | Mouse IgG1, kappa |
Conjugation | Unconjugated |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Purity | Protein A/G affinity |
Immunogen | Amino acids 98-106 (YPYDVPDYA) were used as the immunogen for the HA Tag antibody. |
UniProt ID | Not Known |
Tested applications | ELISA, FACS, IF, IHC-P, IP, WB |
Dilution range | ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml |
Application notes | Optimal dilution of the HA Tag antibody should be determined by the researcher. |
Antibody Type | Primary Antibody |
Clone Number | HA/279 |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Hazard Information | This HA Tag antibody is available for research use only. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE analysis of purified, BSA-free HA Tag antibody (clone HA/279) as confirmation of integrity and purity.
ELISA, ICC, IP, WB | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, FC, IF, IP, WB | |
All | |
Mouse | |
Monoclonal | |
Unconjugated |