H4K8ac Antibody

• Catalog Number: orb1256500
• Category: Antibodies
• Description: H4K8ac Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human Acetyl-Histone H4-K8
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:20 - 1:50CHIP seq: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 11kDa
• Target: H4K8ac
• UniProt ID: P62805
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G,
• Note: For research use only
• Application notes: WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:20 - 1:50CHIP seq: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (orb1256500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat ovary using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (orb1256500). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H4K8ac antibody (orb1256500) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.