H4K5AC Antibody

• Catalog Number: orb1259609
• Category: Antibodies
• Description: H4K5AC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 11kDa
• Target: H4K5AC
• UniProt ID: P62805
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (orb1259609) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).