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Catalog Number | orb1258177 |
---|---|
Category | Antibodies |
Description | H4K20me2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Other, Rat |
Isotype | IgG |
Immunogen | A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 13kDa |
Target | H4K20me2 |
UniProt ID | P62805 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H4-K20 antibody (orb1258177). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H4-K20 antibody (orb1258177).
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H4-K20 antibody (orb1258177) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H4-K20 antibody (orb1258177) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H4-K20 antibody (orb1258177). Blue: DAPI for nuclear staining.
ChIP, ELISA, IF, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, IF, IHC, IP, WB | |
Human, Mouse, Other, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, DOT, IF, IHC, WB | |
C. elegans, Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, IF, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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