H3R17me2a Antibody

• Catalog Number: orb1258169
• Category: Antibodies
• Description: H3R17me2a Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Isotype: IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa
• Target: H3R17me2a
• UniProt ID: P68431
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody (orb1258169). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (orb1258169).

Immunohistochemistry of paraffin-embedded rat lung using Asymmetric DiMethyl-Histone H3-R17 antibody (orb1258169) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using Asymmetric DiMethyl-Histone H3-R17 antibody (orb1258169) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using Asymmetric DiMethyl-Histone H3-R17 antibody (orb1258169) at dilution of 1:200 (40x lens).