H3K27ac Antibody

• Catalog Number: orb1256505
• Category: Antibodies
• Description: H3K27ac Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 16kDa
• Target: H3K27ac
• UniProt ID: P68431
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (orb1256506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody (orb1256506) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.