H3F3A Antibody

SKU: orb418607

Description

This H3F3A (Ab-79) Antibody is an unconjugated polyclonal product. It tagets H3F3A using a Peptide sequence around site of Lys (79) derived from Human Histone H3.3 as the immunogen. This antibody is suitable for ChIP, ELISA, WB. Purification: Antigen Affinity Purified.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ChIP, ELISA, WB
• Dilution range: WB:1:500-5000
• Reactivity: Human, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Peptide sequence around site of Lys (79) derived from Human Histone H3.3
• Target: H3F3A
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Disclaimer: For research use only

Alternative Names

Anti-Histone H3.3 antibody, anti- H3F3A antibody, anti- H3.3A antibody, anti- H3F3 antibody, anti- PP781 antibody, anti- AND antibody, anti- H3F3B antibody, anti- H3.3B antibody

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- H3F3A (Ab-79) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat spleen tissueusing H3F3A (Ab-79) antibody

Quick Database Links

Gene Symbol

H3F3A

UniProt

P84243