H3F3A (Ab-79) antibody

• Catalog Number: orb418607
• Category: Antibodies
• Description: H3F3A (Ab-79) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Peptide sequence around site of Lys (79) derived from Human Histone H3.3
• Dilution range: WB:1:500-5000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: H3F3A
• UniProt ID: P84243
• Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: Anti-Histone H3.3 antibody, anti- H3F3A antibody,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- H3F3A (Ab-79) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Rat spleen tissueusing H3F3A (Ab-79) antibody