H2-Q10 Rabbit Polyclonal Antibody

SKU: orb1172443

Description

H2-Q10 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant mouse H2-Q10 protein
• Target: H2-Q10
• Molecular Weight: 37 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H-2 class I histocompatibility antigen, Q10 alpha chain; HA10_MOUSE;

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-H2-Q10 antibody (orb1172443), Dilution: 1 ug/Test, Secondary Antibody (green line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Negative control (white blue line): PBS, Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-H2-Q10 antibody (orb1172443), Dilution: 1 ug/Test, Secondary Antibody (green line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Negative control (white blue line): PBS, Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (orb1172443) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (orb1172443) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Testis tissue lysates, Primary: Anti-H2-Q10 (orb1172443) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kDa, Observed band size: 37 kDa.

Quick Database Links

Gene Symbol

H2-Q10

UniProt

P01898