H-Ras antibody

• Catalog Number: orb43873
• Category: Antibodies
• Description: Mouse Monoclonal to H-Ras.
• Clonality: Monoclonal
• Clone Number: H-RAS-03
• Tested applications: WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: H-RAS
• Entrez: 3265
• UniProt ID: P01112
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-H-Ras antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human H-Ras using mouse monoclonal antibody H-Ras-03 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for H-Ras at approximately 19 kDa.