Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347087
• Category: Antibodies
• Description: Guinea Pig IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: AP
• Reactivity: Guinea pig
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Purity: Anti-GUINEA PIG IgG (H&L) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum.
• Immunogen: Guinea Pig IgG whole molecule
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:5,000 - 1:15,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Application notes: Anti-GUINEA PIG IgG alkaline phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: rabbit Anti-Guinea Pig IgG Antibody alkaline phosp
• Note: For research use only

ELISA Results of Rabbit Anti-Guinea Pig IgG Antibody Alkaline Phosphatase Conjugated tested against purified Guinea Pig IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n orb2652762). The working dilution is 1:14500. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate and NPP Working Buffer.