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Catalog Number | orb1953429 |
---|---|
Category | Proteins |
Description | GUCY2C Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.78 kDa and the accession number is P25092-1. |
Tag | C-His |
Purity | 98.00% |
MW | 47.78 kDa (predicted); 72.11 kDa (reducing conditions) |
UniProt ID | P25092 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | GUCY2C Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.78 kDa and the accession number is P25092-1. |
Expression Region | A DNA sequence encoding the human GUCY2C (P25092-1) (Met1-Gln430) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Ser 24 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46.8 kDa after removal of the signal peptide. The apparent molecular mass of GUCY2C-His is approximately 55-130 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result. |
98.00% | |
65-110 KDa (reducing condition) |
98.00% | |
48.8 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human Guanylyl Cyclase C/GUCY2C Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser24-Gln430) of Human Guanylyl Cyclase C/GUCY2C fused with His Tag and Avi tag at the C-terminal. |