GTF3C5 Rabbit Polyclonal Antibody

SKU: orb183967

Description

GTF3C5 Rabbit Polyclonal Antibody

Research Area

DNA and RNA, DNA and RNA Research, RNA polymerase III, Transcription Factors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GTF3C5 (331-430/519aa)
• Target: GTF3C5
• Molecular Weight: 59 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TFIIIC63; TFIIICepsilon; TFiiiC2-63; CK820_G0012124; 2700084A09Rik; Q5T7U2_HUMAN; Q5T7U3_HUMAN; TF3C5_HUMAN; GTF3C5; TF3C-epsilon; Transcription factor IIIC 63 kDa subunit (TFIIIC 63 kDa subunit | TFIIIC63); Transcription factor IIIC subunit epsilon; TF3C5_MOUSE; general transcription factor IIIC subunit 5; general transcription factor IIIC, polypeptide 5 (63kD); general transcription factor IIIC, polypeptide 5, 63kDa; transcription factor IIIC, 63 kD

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GTF3C5) Polyclonal Antibody, Unconjugated (orb183967) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-GTF3C5 (orb183967) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 59 kD.

Quick Database Links

Gene Symbol

GTF3C5

UniProt

Q5T7U2