GTF2H3 Antibody

• Catalog Number: orb1256540
• Category: Antibodies
• Description: GTF2H3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human GTF2H3 (NP_001507.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 36kDa
• Target: GTF2H3
• UniProt ID: Q13889
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: GTranscription Factor Antibodies2H3, P34, BTF2, TF
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using GTF2H3 antibody (orb1256540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of A549 cells using GTF2H3 antibody (orb1256540). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HT-29 cells using 1 ug GTF2H3 antibody (orb1256540). Western blot was performed from the immunoprecipitate using GTF2H3 antibody (orb1256540) at a dilution of 1:1000.