GSTM3 Antibody / Glutathione S-Transferase mu 3

• Catalog Number: orb1824615
• Category: Antibodies
• Description: Members of the glutathione S-transferase (GST) family of proteins function in the detoxification of xenobiotics to protect cells against toxicant-induced damage. There are eight families of GST proteins, each of which are composed of proteins that have a variety of functions throughout the cell. The GSTM proteins (GSTM1-GSTM5 in human and GSTM1-GSTM7 in mouse) are members of the mu class of enzymes that conjugate with glutathione and function in the detoxification of carcinogens, environmental toxins and products of oxidative stress. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. GSTM3 protein is selectively expressed in the testis and brain. GSTM3 may also be tumor suppressive in RCC, as low expression has been demonstrated to correlate with RCC risk and poor prognosis in post-op patients.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rGSTM3/8878
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human GSTM3 protein was used as the immunogen for the recombinant GSTM3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant GSTM3 antibody is available for research use only.
• UniProt ID: P21266
• Storage: Aliquot the recombinant GSTM3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney tissue with recombinant GSTM3 antibody (clone rGSTM3/8878). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant GSTM3 antibody (clone rGSTM3/8878) as confirmation of integrity and purity.

Western blot testing of human T-47D cell lysate with recombinant GSTM3 antibody (clone rGSTM3/8878).