GSS antibody

• Catalog Number: orb378090
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GSS.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Glutathione Synthetase
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GSS
• Entrez: 2937, 14854, 25458
• UniProt ID: P46413, P51855, P48637
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Glutathione synthetase; GSH synthetase; GSH-S; Glu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Glutathione Synthetase expression in SW480 (A), K562 (B), Raji (C), mouse kidney (D), mouse liver (E) whole cell lysates. (Predicted band size: 40; 52 kD; Observed band size: 52 kD)

Immunofluorescent analysis of Glutathione Synthetase staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.