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Catalog Number | orb1263600 |
---|---|
Category | Antibodies |
Description | GSS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Predicted Reactivity | Bovine, Monkey, Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This GSS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the C-terminal region of human GSS. |
Concentration | batch dependent |
Dilution range | For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 52 kDa |
Target | GSS |
UniProt ID | P48637 |
NCBI | P48637 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Glutathione synthetase, GSH synthetase, GSH-S, Glu Read more... |
Note | For research use only |
Application notes | For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody. Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of U-87 MG cells stained with GSS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis in Hela, 293T, MDA-MB-453 cell line and mouse liver tissue lysates (35 ug/lane).
Western blot analysis in MDA-MB453 cell line lysates (35 ug/lane).
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