GSK3 Alpha (phospho-S21) antibody

• Catalog Number: orb345419
• Category: Antibodies
• Description: GSK3 Alpha (phospho-S21) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.
• Concentration: 1.28 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IF: User Optimized, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against the phosphorylated form of human GSK3A at the pS 21 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100% homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P49840
• NCBI: 49574532
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-GSK3 alpha pS21 antibody, rabbit anti-
• Note: For research use only
• Application notes: This affinity-purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract. Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS21 of GSK3A.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human derived 293T cell extracts using GSK3 Alpha (phospho-S21) antibody