GSK-3 beta Mouse Polyclonal Antibody

• Catalog Number: orb10756
• Category: Antibodies
• Description: GSK-3 beta Mouse Polyclonal Antibody
• Species/Host: Mouse
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rat, Sheep, Zebrafish
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GSK-3 Beta (1-50/420aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: GSK3B
• UniProt ID: P49841
• RRID: AB_10751530
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Glycogen synthase kinase-3 beta; glycogen synthase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 beta) Polyclonal Antibody, Unconjugated (orb10756) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Brain (mouse) Lysate at 40 ug, Primary: Anti-GSK-3 beta (orb10756) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 47kD, Observed band size: 48kD.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (GSK-3 beta) polyclonal Antibody, Unconjugated (orb10756) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (GSK-3 beta) polyclonal Antibody, Unconjugated (orb10756) 1:100, 90 minutes at 37°C, followed by a CY5 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.