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Catalog Number | orb183938 |
---|---|
Category | Antibodies |
Description | GSDMC Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human GSDMC (1-100/508aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 58 kDa |
Target | GSDMC |
UniProt ID | Q9BYG8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Gasdermin C; Gasdermin-C; GSDMC; GSDMC_HUMAN; Mela Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSDMC) Polyclonal Antibody, Unconjugated (orb183938) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: EC-9706 Cell (Human) Lysate at 40 ug, FHC Cell (Human) Lysate at 40 ug, Primary: Anti-GSDMC (orb183938) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.
Sample: Lane 1: Human A431 cell lysates, Lane 2: Human A549 cell lysates, Lane 3: Human 293T cell lysates, Primary: Anti-GSDMC (orb183938) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kDa, Observed band size: 47 kDa.
ELISA, IHC, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |