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Catalog Number | orb27555 |
---|---|
Category | Antibodies |
Description | GRP94/HSP90B1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, IHC-Fr, WB |
Predicted Reactivity | Bovine |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human GRP94 (687-700aa INPRHPLIRDMLRR), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat Immunocytochemistry , 0.5-1μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 92469 MW |
UniProt ID | P14625 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Endoplasmin;94 kDa glucose-regulated protein;GRP-9 Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Immunocytochemical staining of paraffin embedded MCF-7 cell using HSP90 beta antibody
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Human | |
Rat | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Canine, Equine, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep | |
Rat | |
Monoclonal | |
Unconjugated |
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