GRP78 (Bip) Antibody

• Catalog Number: orb1822422
• Category: Antibodies
• Description: Rabbit Anti-Human GRP78 (Bip) Polyclonal
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Canine, Drosophila, Hamster, Human, Mouse, Rat
• Immunogen: Full length human GRP78 (Bip) his tagged at the N terminus
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Conjugation: Unconjugated
• MW: 78kDa
• Target: GRP78
• Entrez: 3309
• UniProt ID: P11021
• NCBI: NP_005338.1
• Storage: -20°C
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: 78 kDa glucose regulated protein Antibody, 78 kDa
• Note: For research use only
• Application notes: 0.5 µg/ml of SPC-180 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 (Bip) Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 (Bip) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:1000.