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GRP75 Rabbit Polyclonal Antibody

Catalog Number: orb10746

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10746
CategoryAntibodies
DescriptionGRP75 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human GRP75 (561-679/679aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW70 kDa
TargetHSPA9
UniProt IDP38646
RRIDAB_10746576
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesG protein-coupled receptor75; 75 kDa glucose regul
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NoteFor research use only
Expiration Date12 months from date of receipt.
GRP75 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GRP75 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GRP75 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GRP75 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GRP75 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GRP75 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GRP75) Polyclonal Antibody, Unconjugated (orb10746) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GRP75 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Rat Liver tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Rat Cerebrum tissue lysates, Lane 7: Rat Pancreas tissue lysates, Lane 8: Human K562 cell lysates, Lane 9: Human MCF-7 cell lysates, Lane 10: Human Jurkat cell lysates, Lane 11: Human HeLa cell lysates, Lane 12: Human HepG2 cell lysates, Primary: Anti-GRP75 (orb10746) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kDa, Observed band size: 72 kDa.

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