Growth hormone receptor Rabbit Polyclonal Antibody

SKU: orb10712

Description

Growth hormone receptor Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cell Differentiation, Hormone Research

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GHR (101-200/451aa)
• Target: GHR
• Molecular Weight: 68 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GH receptor; GHBP; GHR; Growth hormone receptor precursor; Serum binding protein; Somatotropin receptor; GHR_HUMAN.

Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min). Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 5 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Positive control: A549 cells, Concebtration: 1 µg/10^6 cells, Incubation conditions: Avoid light, 30 minutes on the ice.

Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Testis (Rat) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Growth hormone receptor (orb10712) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130/110 kD, Observed band size: 130 kD.

Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (orb10712) at 1:300 followed by conjugation to a secondary antibody and staining.

Quick Database Links

Gene Symbol

GHR

UniProt

P10912

References

Zhao, Yong et al. Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production Theriogenology, 89, 280-288 (2017)

PubMed: 28043364