Growth Hormone Antibody / GH1

• Catalog Number: orb606947
• Category: Antibodies
• Description: Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly).
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A human partial recombinant protein corresponding to amino acids 58-187 was used as the immunogen for this recombinant GH antibody.
• UniProt ID: P01241
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant GH antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: rGH/1450
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant GH antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human pituitary gland with recombinant GH antibody (clone rGH/1450). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant GH antibody (clone rGH/1450) as confirmation of integrity and purity.