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CXCL2 Antibody
SKU: orb1272519
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO beta (human GRO-beta). |
| Target | CXCL2 |
| Purification | Anti-hGRO beta specific antibody was purified by affinity chromatography employing immobilized hGRO beta matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, GRO2, GROB, C-X-C motif chemokine 2, Growth-regulated protein beta, Gro-beta
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To detect Human GRO-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human GRO-beta (orb1272518) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO-beta.
To detect human GRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect human GRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CXCL2 Antibody (orb1272519)
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