GRHL3 Rabbit Polyclonal Antibody

• Catalog Number: orb577144
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GRHL3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GRHL3
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 57 kDa, 70 kDa
• Target: GRHL3
• UniProt ID: Q8TE85
• Protein Sequence: Synthetic peptide located within the following region: EEVFDALMLKTPDLKGLRNAISEKYGFPEENIYKVYKKCKRGILVNMDNN
• NCBI: NP_937817
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: SOM, VWS2, TFCP2L4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/ml of the antibody was used in this experiment. Several isoforms can be detected using this antibody including isoform 1 (70 kDa) and isoform 4 (57 kDa).

25 ug of the indicated whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Recommended dilution for antibody is 1-3 ug/ml.

Immunohistochemistry of formalin-fixed, paraffin-embedded Human Placenta tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

WB Suggested Anti-GRHL3 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: Jurkat cell lysate.