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Gremlin Rabbit Polyclonal Antibody

Catalog Number: orb500806

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb500806
CategoryAntibodies
DescriptionGremlin Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Gallus, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human gremlin (101-184/184aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW21 kDa
TargetGREM1
UniProt IDO60565
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCKTSF1B1; GREMLIN-1; GREMLIN 1; GREMLIN1; Cysteine
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NoteFor research use only
Expiration Date12 months from date of receipt.
Gremlin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Gremlin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat rectum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Gremlin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Gremlin Rabbit Polyclonal Antibody

Sample: intestine (Mouse) Lysate at 40 ug, skin (Mouse) Lysate at 40 ug, Primary: Anti-Gremlin at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 24 kD.

Gremlin Rabbit Polyclonal Antibody

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Gremlin (orb500806) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Gremlin Rabbit Polyclonal Antibody

Tissue/Cell: Mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated (orb500806) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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