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Catalog Number | orb500806 |
---|---|
Category | Antibodies |
Description | Gremlin Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Bovine, Canine, Equine, Gallus, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human gremlin (101-184/184aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 21 kDa |
Target | GREM1 |
UniProt ID | O60565 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | CKTSF1B1; GREMLIN-1; GREMLIN 1; GREMLIN1; Cysteine Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat rectum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (orb500806) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: intestine (Mouse) Lysate at 40 ug, skin (Mouse) Lysate at 40 ug, Primary: Anti-Gremlin at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 24 kD.
Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Gremlin (orb500806) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.
Tissue/Cell: Mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated (orb500806) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
IHC-P, WB | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Goat, Guinea pig, Human, Rabbit, Zebrafish | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |