Grem1 Antibody

• Catalog Number: orb1263984
• Category: Antibodies
• Description: Grem1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the C-terminal region of mouse GREMLIN.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: Grem1
• UniProt ID: O70326
• NCBI: O70326
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Gremlin-1, Cysteine knot superfamily 1, BMP antago
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-Gremlin Pab in CEM cell line lysates (35 ug/lane)

Western blot analysis of anti-Gremlin Pab in mouse liver cell lysate. Gremlin was detected by using AP6133a.

Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.