GPR91 Rabbit Polyclonal Antibody

• Catalog Number: orb157370
• Category: Antibodies
• Description: GPR91 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GPR91 (131-334/334aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: SUCNR1
• UniProt ID: Q9BXA5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: G protein coupled receptor 91; G-protein coupled r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

W Su, Y Wang, C Zu, L Lei, H Li G protein-coupled receptor 91 promotes the inflammatory response to Porphyromonas gingivalis in bone marrow-derived macrophages Heliyon, (2024)

PubMed:

Applications

IF, IHC, WB

Reactivity

Mouse

W Su, D Zhang, Y Wang, L Lei, H Li G protein-coupled receptor 91 activations suppressed mineralization in Porphyromonas gingivalis–infected osteoblasts researchsquare.com, (2024)

PubMed:

Applications

WB

Reactivity

Mouse

Protein: 1.kidney lyates (mo), 2.liver lyates (mo), Primary: Rabbit Anti-GPR91 (orb157370) at 1:300, Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG (H+L) at 1: 10000, Predicted band size: 39 kD, Observed band size: 49 kD.

Sample: HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-GPR91 (orb157370) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kD, Observed band size: 39kD.

Tissue/Cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (orb157370) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (orb157370) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Western blot analysis of extracts from Liver tissue (mo) using GPR91 Antibody.