GPR49 Rabbit Polyclonal Antibody (PE-Cy5)

SKU: orb899290

Description

GPR49 Rabbit Polyclonal Antibody (PE-Cy5) is a PE/Cy5 conjugated antibody targeting LGR5. This antibody is suitable for FC, IF. It exhibits reactivity with Human, Mouse samples.

Research Area

G Protein-Coupled Receptors, GPCR Signaling Pathway, Molecular Biology, Surface Molecules

Images & Validation

• Tested Applications: FC, IF
• Dilution range: Flow-Cyt=3μg/Test, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse GPR49 (810-907/907aa)
• Target: LGR5
• Purification: Affinity purified by Protein A
• Conjugation: PE/Cy5

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

LGR5; G protein coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; GPCR GPR49; G protein coupled receptor 67; FEX; g protein-coupled receptor fex; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; Leucine rich repeat containing G protein coupled receptor 5; HG 38; HG38; LGR5_HUMAN; MGC117008; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; Orphan G protein coupled receptor HG38.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CD44 antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal rabbit serum) at 37°C for 20 min, Incubation: Anti-GPR49 Polyclonal Antibody, conjugated (orb899290) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

LGR5