GPR43 Rabbit Polyclonal Antibody

• Catalog Number: orb159222
• Category: Antibodies
• Description: GPR43 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GPR43 (41-140/330aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: FFAR2
• UniProt ID: O15552
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GPCR GPR43; FFA2R; Ffar2; FFAR2_HUMAN; free fatty
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

He Yu, Lijun Cui, Xiaomei Wang, Xu Wang, Shaoyang Liu, Chen Bai Effect of fecal microbiota transplantation on “intestinal flora-SCFAs-GPR43 - gastrointestinal peptide” pathway in rats with high-fat diet researchsquare.com, (2025)

PubMed:

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (GPR43) polyclonal Antibody, Unconjugated (orb159222) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GPR43 Polyclonal Antibody, Unconjugated (orb159222) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.