GPR4 Rabbit Polyclonal Antibody

• Catalog Number: orb157336
• Category: Antibodies
• Description: GPR4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human G protein coupled receptor 4 (201-300/362aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: GPR4
• UniProt ID: P46093
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: G protein coupled receptor 4; G-protein coupled re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-GPR19/GPR4 antibody (orb157336), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-GPR4 (orb157336) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 51 kD.

Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-GPR4 (orb157336) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 43 kD.