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Catalog Number | orb1600538 |
---|---|
Category | Antibodies |
Description | GPR15 Rabbit Polyclonal Antibody (BF488) |
Target | GPR15 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | BF488 |
Reactivity | Human, Mouse, Rat |
Predicted Reactivity | Canine, Equine, Rabbit |
Concentration | 1mg/ml |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide derived from human GPR15 (51-150/360aa) |
Tested applications | IF |
Dilution range | IF=1:200-800 |
Antibody Type | Primary Antibody |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Alternative names | GPCR GPR15; BOB; G protein coupled receptor 15; G Read more... |
Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GPR15 Antibody, conjugated (orb1600538) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.