GPR110 Rabbit Polyclonal Antibody

• Catalog Number: orb157302
• Category: Antibodies
• Description: GPR110 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human G protein coupled receptor 110 (51-150/910aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 99 kDa
• Target: ADGRF1
• UniProt ID: Q5T601
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: G protein coupled receptor 110; G protein coupled
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Mouse Kidney Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-GPR110 antibody (orb157302), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: A431 (human) cell Lysate at 40 ug, Primary: Anti-GPR110 (orb157302) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 99 kD, Observed band size: 109 kD.

Sample: A549 (human) cell Lysate at 40 ug, Primary: Anti-GPR110 (orb157302) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 99 kD, Observed band size: 109 kD.