GPM6B Rabbit Polyclonal Antibody

• Catalog Number: orb157286
• Category: Antibodies
• Description: GPM6B Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GPM6B (1-100/265aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: GPM6B
• UniProt ID: Q13491
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: M6b; Neuronal membrane glycoprotein M6b; rhombex 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-GPM6B (orb157286) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 36 kD.

Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-GPM6B (orb157286) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 34 kD.