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Catalog Number | orb1249397 |
---|---|
Category | Antibodies |
Description | GPI Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-YREHRSELNLRR |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Heart lysates (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was hour.Immunohistochemistry:Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | GPI |
UniProt ID | P06744 |
NCBI | NP_000166.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | GPI, glucose phosphate isomerase, AMF, GNPI, NLK, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Heart lysates (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was hour.Immunohistochemistry:Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249397 (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249397 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1249397 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1249397 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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