GPI/Neuroleukin Antibody

• Catalog Number: orb1248598
• Category: Antibodies
• Description: GPI/Neuroleukin Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RERMFNGEKINYT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Heart lysates and approx 55kDa observed in lysates of cell lines HeLa, A431, A549, MCF7, Jurkat and K562 (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 60kDa
• Target: GPI / Neuroleukin
• UniProt ID: P06744
• NCBI: NP_000166.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GPI, glucose phosphate isomerase, AMF, GNPI, NLK,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Heart lysates and approx 55kDa observed in lysates of cell lines HeLa, A431, A549, MCF7, Jurkat and K562 (calculated MW of 63.1kDa according to NP_000166.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis, Thyroid and Kidney. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248598 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248598 (0.05 ug/ml) staining of HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E) and K562 (F) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

orb1248598 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.